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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Hortikultura Indonesia Buletin Hama dan Penyakit Tumbuhan Zuriat MIMBAR (Jurnal Sosial dan Pembangunan) Jurnal Natur Indonesia Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar Wacana, Jurnal Sosial dan Humaniora Jurnal RISET Geologi dan Pertambangan AGRIVITA, Journal of Agricultural Science Jurnal NERS Jurnal Agroekoteknologi International Journal of Sustainable Tropical Agricultural Sciences Indonesian Journal of Biotechnology UNEJ e-Proceeding Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Constitutional Review ICoTE Proceedings Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Penelitian Kelapa Sawit Journal of Tropical Crop Science Menara Perkebunan Berkala Penelitian Agronomi Journal of English Language Teaching Innovations and Materials (Jeltim) JIIP (Jurnal Ilmiah Ilmu Pendidikan) Jurnal Tanah dan Iklim Jurnal Agrotropika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) Journal of General-Procedural Dermatology & Venereology Indonesia Jurnal Edukasi Biologi
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Departemen Agronomi Dan Hortikultura Fakultas Pertanian, Institut Pertanian Bogor
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Journal : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

 1 
TRANSGENE IDENTITY AND NUMBER OF INTEGRATION SITES AND THEIR CORRELATION WITH RESISTANCE TO PStV IN TRANSGENIC PEANUTS CARRYING PEANUT STRIPE VIRUS (PStV) COAT PROTEIN GENE Dwi Hapsoro, Hajrial Aswidinnoor, Rusmilah Suseno, Jumanto, and Sudarsono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 1 (2007): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.151 KB) | DOI: 10.23960/j.hptt.17%p

Abstract

Transgene Identity and Number of Integration Sites and Their Correlation with Resistance To PStV in Transgenic Peanuts Carrying Peanut Stripe Virus (PStV) Coat Protein Gene. This research aimed to determine (1) the identity and copy number of PStV cp gene in transgenic peanut plants carrying PStV cp gene and (2) correlation between the identity and the number of integration sites and resistance to PStV infection. One T0 transgenic peanut was selfed up to five generations. T2, T3, and T5 plants were mechanically inoculated with PStV. Samples of T5 plants derived from several different T4 plants were subjected to Southern analysis to confirm the integration of PStV cp gene and to determine its identity and copy number. The Southern analysis showed three bands of different size, i.e. 1.1 kb, 1.3 kb, and 5.8 kb. Most of the lines of T5 generation have one insertion site, suggesting that the three insertion sites were located in different loci. Based on the phenotypic data, the transgenes of 1.1 kb and 1.3 kb were functional, resulting in resistant or recovery phenotype, while that of 5.8 kb was not functional. Copy number apparently had no effects on the phenotypes.
IDENTIFIKASI DINI GALUR KACANG TANAH YANG RESISTEN TERHADAP INFEKSI SCLEROTIUM ROLFSII HASIL SELEKSI IN VITRO DENGAN FILTRAT KULTUR S. ROLFSII Yusnita, Widodo, Rita Megia, Hajrial Aswidinnoor dan Sudarsono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 6 No. 1 (2006): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.891 KB) | DOI: 10.23960/j.hptt.1641-51

Abstract

Early Identification of Sclerotium rolfsii Resistant Lines of Peanut Derived from In Vitro Selection Using S. rolfsii Culture Filtrates. The objectives of this experiment was to evaluate methods for early identification of Sclerotium rolfsii resistant lines derived from fungal culture filtrate (CF)-insensitive somatic embryos (SE) and to correlate the results of early identification with that of direct S. rolfsii inoculation in the plastic house. In the first method, shoots regenerated from CF-insensitive SE were planted onto selective medium containing 30% of S. rolfsii CF for one month and then responses were evaluated. In the second method, leaves and leaflets of R0 peanut lines derived from CF-insensitive SE were inoculated with S. rolfsii by detached leaf-dual culture test. The damages of the inoculated leaves and leaflets were assessed 6 days after inoculation. A number of R1 plants were grown in the plastic house from seeds harvested from R0 peanut lines, and they were inoculated with S. rolfsii at 30 days after planting (dap). Responses of the inoculated R1 plants were observed at 44 dap. Results of the experiment indicated that shoots from CF-insensitive SE showed less damage scores (DS=1.2), when cultured on CF-containing selective medium than ones from non-selected SE (DS=2.9). Most of the detached leaves and leaflets inoculated with S. rolfsii totally necrosed. However, leaves and leaflets from 7 out of 23 R0 lines showed variegation between necrosed and healthy tissues, indicating the presence of S. rolfsii resistant tissue among the tested leaves and leaflets. Direct inoculation of R1 plants in the plastic house showed 10 out of 18 progenies derived from 6 R0 lines showed less disease severity scores (DSS=2 or 3) and they were able to survive and produce seeds. On the other hand, seed derived (original) peanut plants cv. Kelinci inoculated with S. rolfsii all showed DSS=5 and died after inoculation. The overall results of the experiment indicated the usefullness of in vitro selection using S. rolfsii culture filtrates for regenerating S. rolfsii resistant peanut variants and the possible use of methods for early identification of desirable variants.
VARIAN SOMAKLONAL KACANG TANAH RESISTEN SCLEROTIUM ROLFSII HASIL SELEKSI IN VITRO MENGGUNAKAN FILTRAT KULTUR CENDAWAN Yusnita, Hajrial Aswidinnoor, Rusmilah Suseno & Sudarsono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 1 (2010): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.605 KB) | DOI: 10.23960/j.hptt.11035-46

Abstract

Peanut somaclonal variants resistant to Sclerotium rolfsii derived from in vitro selection with fungal culture filtrates. Sclerotium stem rot is one of the most important peanut disease which often caused significant yield loss. The use of peanut cultivars resistant to Sclerotium rolfsii infection is the most efficient way to control the disease. Attempts to obtain peanut lines with tolerance or resistance to Sclerotium stem rot through induction of somaclonal variation and in vitro selection using fungal culture filtrates (CF) have been conducted previously. Somatic embryo (SE) clumps that had been maitained in culture for approximately one year in regeneration medium were exposed onto selective medium containing 30% S. rolfsii CF for three consecutive 1 month –passages, and the SE formed after selection periods were considered to be insensitive to the fungal CF. A number R0 peanut lines have been regenerated from CF-insensitive SE, and their R1 and R2 progenies were grown in a plastic house for evaluation on qualitative and quantitative variant characters. Early identification for tolerance to S. rolfsii was also conducted among R0 peanut lines, and the results have shown enhanced resistance when compared to the original non-selected cultivar. However, further evaluation is needed to study responses of the R1 and R2 progenies of the somaclones against S. rolfsii infection. The objective of this particular study was to evaluate responses of R1 and R2 peanut somaclones derived from fungal CF-insensitif SE against S. rolfsii infection in the plastic house. Results of this experiment showed, a number of resistant somaclonal variants were obtained among R1 and R2 population, which segregated as resistance and susceptible to S. rolfsii infection. The resistance somaclonal variants initially showed stem rot symptoms after inoculation with S. rolfsii with disease score (DS) of 1 to 3. However, as the plants grew and developed, they showed wound-healing process at the lesio and they were able to produce the same or higher number of filled pod as the original non-inoculated peanut plants.
INHERITANCE OF RESISTANCE TO PStV IN TRANSGENIC PEANUTS CONTAINING cp PStV GENE Dwi Hapsoro, Hajrial Aswidinnoor, Rusmilah Suseno, Jumanto, dan Sudarsono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 8 No. 1 (2008): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.136 KB) | DOI: 10.23960/j.hptt.1831-38

Abstract

Inheritance of Resistance to PStV in Transgenic Peanuts Containing cp PStV Gene. We have obtained transgenic peanut lines containing coat protein gene of PStV. To get maximal use of the transgenic character in a breeding program, it is necessary that the transgene is also stably inherited and expressed. This experiment was conducted from June 2002 – January 2005 at Plant Molecular Biology Laboratory, Bogor Agricultural University, and Queensland Agricultural Biotechnology Center, The University of Queensland, Australia. The research aimed (1) to test whether PStV cp transgene was functional in progenies derived from crosses between transgenic peaanut plants containing PStV cp gene and non-transgenic ones and (2) to determine pattern of inheritance of resistance to PStV as a result of PStV cp gene action. Several crosses were made between trangenic peanut cv.Gajah resistant to PStV (T4 generation) and non-transgenic peanut line WS susceptible to PStV. The F1 and F2 populations were mechanically inoculated with PStV two weeks after planting. The experiment showed that all plants in the F1 population were less susceptible to PStV, suggesting that the transgene was partially dominant. Phenotipic segregation in F2 population was not Mendelian with the appearance of quick and slow recovery plants and the number of resistant plants being more than expected. However, the proportion of transgenic and non-transgenic plants followed 3:1 ratio, which was Mendelian.
Co-Authors , Agustiansyah , Rustikawati , Sukendah , Triadiati , Witjaksono ABDUL RACHMAD BUDIONO Ade Wachjar Adisyahputra Adisyahputra, Adisyahputra Agung Endro Nugroho Agus Purwito Agus Rachmat AHMAD RIDUAN Ahmad Surkati Ali Husni Amarillis, Shandra Amarullah, Muhamad Roffi Andrie Al Kausar Anita Yuliyanti Aniversari Apriana Anneke Pesik Anwar, Aswaldi Aprizal Zainal Arfan Nazhri Simamora Arief Hidayat Aris Munandar Asep Setiawan ASMONO, DWI Atmitri Sisharmini Bambang Heliyanto Bambang S. Purwoko BAMBANG SUGIRI Barahima Abbas C J Soegihardjo Cartealy, Imam Civi Cartealy, Imam Civi Catur Herison Cecep Kusmana Christoph Leuschner Clarry Sada Colleen M. Higgins, Colleen M. Darmawan Saptadi Deka Rotama Dewi Sukma Didiek Hadjar Goenadi Diky Anggoro DINARTY, DINY Dini Dinarty, Dini DINI NURDIANI Diny Dinarti Djoko Santoso DWI ANDREAS SANTOSA Dwi Asmono DWI HAPSORO Dwi Hapsoro Edi Guhardja Efendi, Darda Elina, Juanita ELSJE T. TENDA, ELSJE T. Endang Pudjihartati Endrizal Eneng Fakhrunnisa Enni Suwarsi Rahayu Erick Raynalta, Erick Fajar Laksono Suroso Fakhrunnisa, Eneng Fatsan, Ahmad FETRINA OKTAVIA Germini Alam Giyanto . Gusti Ayu Kade Sutariati H. M.H. Bintoro Djoefrie HAJRIAL ASWIDINNOOR Harsini ,, Harsini Hartati, Raden Roro Sri Haryadi Permana Hasriadi Mat Akin Hengky Novarianto Hengky Novarianto Hidayat Pawitan Hiroshi Ehara Hugo Volkaert, Hugo I MADE ARTIKA IBNUL QAYIM IDA HANARIDA SOMANTRI Iim Fatimah Ika Mariska Ikhsanudin Ikhsanudin Irman Firmansyah Ismail Maskromo Ismail Maskromo Iwa Sutardjo, Iwa Iwan Setiawan JAJAH KOSWARA Juang Gema Kartika Jumanto Jumanto Jumanto Jumanto, Jumanto Khoerul Anwar Khursatul Munibah Kukuh Murtilaksono Kukuh Setiawan Kurniasari, Praharisti Kurniawan R. Trijatmiko Kusumo Nugroho Kuswanhadi Kuswanhadi, Kuswanhadi Laksmita Prima Santi Laksono Trisnantoro Lila Yuwana Lollie Agustina P. Putri M. Herman Marbelisa Briliani Mardikaningsih, Agusti Marenda Ishak S MASKROMO, ISMAIL MEITY A. TULALO, MEITY A. Memen Surahman Meynarti Sari Dewi Ibrahim Muchammad Ali Safaat Muhammad Hasyim Bintoro MUHAMMAD HERMAN Muhammad Herman Muhammad Machmud Muhammad Machmud Mutia Dewi Yuniati Neviana Wahyuni Nihayati, Hanik Endang Niniet Indah Arvitrida Noor Cahaya NOVARIANTO, HENGKY Nur Khayati Nurhajati A. Mattjik Nursalam Nursalam Nurul Khumaida Paulus B.K. Santoso Purwaningsih Purwaningsih Purwoko, Devit purwoko, devit R B Gobel Ralf G. Dietzgen, Ralf G. Reni Indrayanti ROEDHY POERWANTO Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati Rubiyo Rubiyo RULLY DYAH PURWATI RUSMILAH SUSENO S. Arsyad S. Ilyas Said Harran Saiful Akhyar Lubis SATRIYAS ILYAS Satriyas Ilyas Satya Nugroho Shinta Hadiyantina Sholeh Avivi Siswanto Siswanto Siti Mufidah Siti Sunarintyas SOEKISMAN TJITROSEMITO Sri H. Hidayat Sri H. Hidayat Sri H. Hidayat Sri Indarto Sri S. Harjadi Sri Soenarsih DAS Stephanie Wilamarta Sudari, Sudari Sudibyo Martono Sudirman Yahya SUDJINDRO SUDJINDRO Sugiyanta Sukendah S, Sukendah SUKENDAH SUKENDAH, SUKENDAH Sumali Wiryowidagdo Suryo Wiyono Susilo Indrawati Swastika, Maria Syafaruddin Syafaruddin TENDA, ELSJE T. Teuku Tajuddin TINCHE, nFn Tri J. Santoso Tri J. Santoso Tri Joko Santoso Tri Joko Santoso U.S WIRADISASTRA UJANG KHAIRUL Urai Salam Visia Assyafira Suwarto vyta andri setyo utami Wening Enggarini Widiatmaka . Widiatmaka Widiatmaka Widodo Widodo Wiwin Ambarwulan Yudiwanti Wahyu E. K. Yulis Setiya Dewi Yundari, Yundari Yuni Wibowo Yusnita Yusnita